| Id: | TF_ChIP-seq/ENCSR706OJM |
| Type: | position_score |
| Version: | 0 |
| Summary: |
TFChIP-seq ENCSR706OJM [biosamplesummary="Homo sapiens SK-N-SH genetically modified\ \ (insertion) using CRISPR targeting H. sapiens POU6F1 treated with 6 \u03BCM\ \ all-trans-retinoic acid for 48 hours" and target="POU6F1"] |
| Description: |
status: released biological_replicates: Rep 1, Rep 2 summary: treated with 6 μM all-trans-retinoic acid for 48 hours genetically modified (insertion) using CRISPR targeting H. sapiens POU6F1 output_type: IDR thresholded peaks audit_internal_action: Released analysis {ENCAN124ZQW|/analyses/ENCAN124ZQW/} has in progress subobject quality standard {encode4-tf-chip|/quality-standards/encode4-tf-chip/} audit_warning: Processed alignments file {ENCFF597YLH|/files/ENCFF597YLH/} processed by ChIP-seq ENCODE4 v1.8.0 GRCh38 pipeline has 19619054 usable fragments. The minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting POU6F1-human and investigated as a transcription factor is 10 million usable fragments. The recommended value is > 20 million, but > 10 million is acceptable. (See {ENCODE ChIP-seq data standards|/data-standards/chip-seq/} ) audit_warning: Processed alignments file {ENCFF455UET|/files/ENCFF455UET/} processed by ChIP-seq ENCODE4 v1.8.0 GRCh38 pipeline has 18371119 usable fragments. The minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting POU6F1-human and investigated as a transcription factor is 10 million usable fragments. The recommended value is > 20 million, but > 10 million is acceptable. (See {ENCODE ChIP-seq data standards|/data-standards/chip-seq/} ) audit_warning: PBC1 (PCR Bottlenecking Coefficient 1, M1/M_distinct) is the ratio of the number of genomic locations where exactly one read maps uniquely (M1) to the number of genomic locations where some reads map (M_distinct). A PBC1 value in the range 0 - 0.5 is severe bottlenecking, 0.5 - 0.8 is moderate bottlenecking, 0.8 - 0.9 is mild bottlenecking, and > 0.9 is no bottlenecking. PBC1 value > 0.9 is recommended, but > 0.8 is acceptable. ENCODE processed alignments file {ENCFF597YLH|/files/ENCFF597YLH/} processed by ChIP-seq ENCODE4 v1.8.0 GRCh38 pipeline was generated from a library with PBC1 value of 0.80. audit_warning: PBC2 (PCR Bottlenecking Coefficient 2, M1/M2) is the ratio of the number of genomic locations where exactly one read maps uniquely (M1) to the number of genomic locations where two reads map uniquely (M2). A PBC2 value in the range 0 - 1 is severe bottlenecking, 1 - 3 is moderate bottlenecking, 3 - 10 is mild bottlenecking, > 10 is no bottlenecking. PBC2 value > 10 is recommended, but > 3 is acceptable. ENCODE processed alignments file {ENCFF597YLH|/files/ENCFF597YLH/} processed by ChIP-seq ENCODE4 v1.8.0 GRCh38 pipeline was generated from a library with PBC2 value of 4.79. audit_warning: NRF (Non Redundant Fraction) is equal to the result of the division of the number of reads after duplicates removal by the total number of reads. An NRF value in the range 0 - 0.5 is poor complexity, 0.5 - 0.8 is moderate complexity, and > 0.8 high complexity. NRF value > 0.8 is recommended, but > 0.5 is acceptable. ENCODE processed alignments file {ENCFF455UET|/files/ENCFF455UET/} processed by ChIP-seq ENCODE4 v1.8.0 GRCh38 pipeline was generated from a library with NRF value of 0.78. audit_warning: PBC1 (PCR Bottlenecking Coefficient 1, M1/M_distinct) is the ratio of the number of genomic locations where exactly one read maps uniquely (M1) to the number of genomic locations where some reads map (M_distinct). A PBC1 value in the range 0 - 0.5 is severe bottlenecking, 0.5 - 0.8 is moderate bottlenecking, 0.8 - 0.9 is mild bottlenecking, and > 0.9 is no bottlenecking. PBC1 value > 0.9 is recommended, but > 0.8 is acceptable. ENCODE processed alignments file {ENCFF455UET|/files/ENCFF455UET/} processed by ChIP-seq ENCODE4 v1.8.0 GRCh38 pipeline was generated from a library with PBC1 value of 0.77. audit_warning: PBC2 (PCR Bottlenecking Coefficient 2, M1/M2) is the ratio of the number of genomic locations where exactly one read maps uniquely (M1) to the number of genomic locations where two reads map uniquely (M2). A PBC2 value in the range 0 - 1 is severe bottlenecking, 1 - 3 is moderate bottlenecking, 3 - 10 is mild bottlenecking, > 10 is no bottlenecking. PBC2 value > 10 is recommended, but > 3 is acceptable. ENCODE processed alignments file {ENCFF455UET|/files/ENCFF455UET/} processed by ChIP-seq ENCODE4 v1.8.0 GRCh38 pipeline was generated from a library with PBC2 value of 4.28. audit_warning: Replicate concordance in ChIP-seq experiments is measured by calculating IDR values (Irreproducible Discovery Rate). ENCODE processed IDR thresholded peaks files {ENCFF065OBK|/files/ENCFF065OBK/} processed by ChIP-seq ENCODE4 v1.8.0 GRCh38 pipeline have a rescue ratio of 2.98 and a self consistency ratio of 1.05. According to ENCODE standards, having both rescue ratio and self consistency ratio values < 2 is recommended, but having only one of the ratio values < 2 is acceptable. |
| Labels: |
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| ID | Type | Default annotation | Description | Histogram | Range |
|---|---|---|---|---|---|
| TF_ChIP-seq_ENCSR706OJM | float |
TF_ChIP-seq_ENCSR706OJM |
TF_ChIP-seq ENCSR706OJM [biosample_summary="Homo sapiens SK-N-SH genetically modified\ \ (insertion) using CRISPR targeting H. sapiens POU6F1 treated with 6 \u03BCM\ \ all-trans-retinoic acid for 48 hours" and target="POU6F1"]
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[11.8, 289] |
| Filename | Size | md5 |
|---|---|---|
| ENCFF065OBK.bed.gz | 74.16 KB | d16674594e4784e518e008a9d78ab5ea |
| ENCFF065OBK.bed.gz.dvc | 99.0 B | 29c038d1ebc3d4ca980c90110391e1c1 |
| ENCFF065OBK.tabix.bed.gz | 49.76 KB | 8b246d1e46093da3f864d8a651b61248 |
| ENCFF065OBK.tabix.bed.gz.dvc | 105.0 B | 9bcec233471493e68e26b02baaa78b24 |
| ENCFF065OBK.tabix.bed.gz.tbi | 38.1 KB | 69e2904048714f5bb9528b34bd2e3706 |
| ENCFF065OBK.tabix.bed.gz.tbi.dvc | 109.0 B | 88f54bb5de5ab94b47f3533a2abe135b |
| genomic_resource.yaml | 6.79 KB | 250e1f8aa975b908cc43ef57091542ad |
| genomic_resource_original.yaml | 6.56 KB | fa2b47a1f49dd793f28b4cbd9cbc8c03 |
| statistics/ |