type: position_score
table:
  filename: ENCFF456EDK.tabix.bed.gz
  header_mode: none
  zero_based: true
  format: tabix
  chrom:
    column_index: 0
  pos_begin:
    column_index: 1
  pos_end:
    column_index: 2
scores:
- id: TF_ChIP-seq_ENCSR539PMH
  column_index: 3
  type: float
  desc: 'TF_ChIP-seq ENCSR539PMH [biosample_summary="Homo sapiens HepG2 genetically modified (insertion) using CRISPR targeting H. sapiens CENPBD1" and target="CENPBD1"]'
  histogram:
    type: number
    number_of_bins: 100
meta:
  summary: 'TF_ChIP-seq ENCSR539PMH [biosample_summary="Homo sapiens HepG2 genetically modified (insertion) using CRISPR targeting H. sapiens CENPBD1" and target="CENPBD1"]'
  description: '**status**: released


    **biological\_replicates**: Rep 1, Rep 2


    **summary**: genetically modified (insertion) using CRISPR targeting H. sapiens
    CENPBD1


    **output\_type**: IDR thresholded peaks


    **audit\_internal\_action**: Released analysis {ENCAN103QZH|/analyses/ENCAN103QZH/}
    has in progress subobject quality standard {encode4-tf-chip|/quality-standards/encode4-tf-chip/}


    **audit\_warning**: Processed alignments file {ENCFF274HCD|/files/ENCFF274HCD/}
    processed by ChIP-seq ENCODE4 v1.8.0 GRCh38 pipeline has 18014489 usable fragments.
    The minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting
    CENPBD1-human and investigated as a transcription factor is 10 million usable
    fragments. The recommended value is > 20 million, but > 10 million is acceptable.
    (See {ENCODE ChIP-seq data standards|/data-standards/chip-seq/} )


    **audit\_warning**: PBC1 (PCR Bottlenecking Coefficient 1, M1/M\_distinct) is
    the ratio of the number of genomic locations where exactly one read maps uniquely
    (M1) to the number of genomic locations where some reads map (M\_distinct). A
    PBC1 value in the range 0 - 0.5 is severe bottlenecking, 0.5 - 0.8 is moderate
    bottlenecking, 0.8 - 0.9 is mild bottlenecking, and > 0.9 is no bottlenecking.
    PBC1 value > 0.9 is recommended, but > 0.8 is acceptable. ENCODE processed alignments
    file {ENCFF274HCD|/files/ENCFF274HCD/} processed by ChIP-seq ENCODE4 v1.8.0 GRCh38
    pipeline was generated from a library with PBC1 value of 0.86.


    **audit\_warning**: PBC2 (PCR Bottlenecking Coefficient 2, M1/M2) is the ratio
    of the number of genomic locations where exactly one read maps uniquely (M1) to
    the number of genomic locations where two reads map uniquely (M2). A PBC2 value
    in the range 0 - 1 is severe bottlenecking, 1 - 3 is moderate bottlenecking, 3
    - 10 is mild bottlenecking, > 10 is no bottlenecking. PBC2 value > 10 is recommended,
    but > 3 is acceptable. ENCODE processed alignments file {ENCFF274HCD|/files/ENCFF274HCD/}
    processed by ChIP-seq ENCODE4 v1.8.0 GRCh38 pipeline was generated from a library
    with PBC2 value of 6.95.


    **audit\_warning**: PBC1 (PCR Bottlenecking Coefficient 1, M1/M\_distinct) is
    the ratio of the number of genomic locations where exactly one read maps uniquely
    (M1) to the number of genomic locations where some reads map (M\_distinct). A
    PBC1 value in the range 0 - 0.5 is severe bottlenecking, 0.5 - 0.8 is moderate
    bottlenecking, 0.8 - 0.9 is mild bottlenecking, and > 0.9 is no bottlenecking.
    PBC1 value > 0.9 is recommended, but > 0.8 is acceptable. ENCODE processed alignments
    file {ENCFF385KXT|/files/ENCFF385KXT/} processed by ChIP-seq ENCODE4 v1.8.0 GRCh38
    pipeline was generated from a library with PBC1 value of 0.83.


    **audit\_warning**: PBC2 (PCR Bottlenecking Coefficient 2, M1/M2) is the ratio
    of the number of genomic locations where exactly one read maps uniquely (M1) to
    the number of genomic locations where two reads map uniquely (M2). A PBC2 value
    in the range 0 - 1 is severe bottlenecking, 1 - 3 is moderate bottlenecking, 3
    - 10 is mild bottlenecking, > 10 is no bottlenecking. PBC2 value > 10 is recommended,
    but > 3 is acceptable. ENCODE processed alignments file {ENCFF385KXT|/files/ENCFF385KXT/}
    processed by ChIP-seq ENCODE4 v1.8.0 GRCh38 pipeline was generated from a library
    with PBC2 value of 5.67.


    **audit\_warning**: Replicate concordance in ChIP-seq experiments is measured
    by calculating IDR values (Irreproducible Discovery Rate). ENCODE processed IDR
    thresholded peaks files {ENCFF456EDK|/files/ENCFF456EDK/} processed by ChIP-seq
    ENCODE4 v1.8.0 GRCh38 pipeline have a rescue ratio of 1.99 and a self consistency
    ratio of 4.47. According to ENCODE standards, having both rescue ratio and self
    consistency ratio values < 2 is recommended, but having only one of the ratio
    values < 2 is acceptable.'
  labels:
    reference_genome: hg38/genomes/GRCh38-hg38
    accession: ENCSR539PMH
    status: released
    assay_term_name: ChIP-seq
    simple_biosample_summary: genetically modified (insertion) using CRISPR targeting
      H. sapiens CENPBD1
    biosample_summary: Homo sapiens HepG2 genetically modified (insertion) using CRISPR
      targeting H. sapiens CENPBD1
    replication_type: isogenic
    biosample_ontology: /biosample-types/cell_line_EFO_0001187/
    perturbed: false
    doi: 10.17989/ENCSR539PMH
    date_created: '2019-08-20T21:57:11.556236+00:00'
    date_released: '2022-07-15'
    submitter_comment: ''
    target: CENPBD1
