type: position_score
table:
  filename: ENCFF533MNU.tabix.bed.gz
  header_mode: none
  zero_based: true
  format: tabix
  chrom:
    column_index: 0
  pos_begin:
    column_index: 1
  pos_end:
    column_index: 2
scores:
- id: Histone_ChIP-seq_ENCSR474VGQ
  column_index: 3
  type: float
  desc: signal
  histogram:
    type: number
    number_of_bins: 100
meta:
  summary: 'ChIP-seq ENCSR474VGQ [TISSUE: Homo sapiens KMS-11, TARGET: H3K27me3]'
  description: "**status**: released\n\n**biological\\_replicates**: Rep 1, Rep 2\n\
    \n**summary**: \n\n**output\\_type**: replicated peaks\n\n**audit\\_internal\\\
    _action**: Archived analysis {ENCAN867VXR|/analyses/ENCAN867VXR/} has in progress\
    \ subobject quality standard {encode3-histone-chip|/quality-standards/encode3-histone-chip/}\n\
    \n**audit\\_not\\_compliant**: Processed alignments file {ENCFF994CYR|/files/ENCFF994CYR/}\
    \ processed by ChIP-seq ENCODE3 hg19 pipeline has 14172563 usable fragments. The\
    \ minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting\
    \ H3K27me3-human and investigated as a broad histone mark is 20 million usable\
    \ fragments. The recommended value is > 45 million, but > 35 million is acceptable.\
    \ (See {ENCODE ChIP-seq data standards|/data-standards/chip-seq/})\n\n**audit\\\
    _warning**: Processed alignments file {ENCFF517FQP|/files/ENCFF517FQP/} processed\
    \ by ChIP-seq ENCODE3 hg19 pipeline has 39755015 usable fragments. The minimum\
    \ ENCODE standard for each replicate in a ChIP-seq experiment targeting H3K27me3-human\
    \ and investigated as a broad histone mark is 20 million usable fragments. The\
    \ recommended value is > 45 million, but > 35 million is acceptable. (See {ENCODE\
    \ ChIP-seq data standards|/data-standards/chip-seq/})\n\n**audit\\_warning**:\
    \ NRF (Non Redundant Fraction) is equal to the result of the division of the number\
    \ of reads after duplicates removal by the total number of reads. An NRF value\
    \ in the range 0 - 0.5 is poor complexity, 0.5 - 0.8 is moderate complexity, and\
    \ > 0.8 high complexity. NRF value > 0.8 is recommended, but > 0.5 is acceptable.\
    \ ENCODE processed alignments file {ENCFF994CYR|/files/ENCFF994CYR/} processed\
    \ by ChIP-seq ENCODE3 hg19 pipeline was generated from a library with NRF value\
    \ of 0.63.\n\n**audit\\_warning**: PBC1 (PCR Bottlenecking Coefficient 1, M1/M\\\
    _distinct) is the ratio of the number of genomic locations where exactly one read\
    \ maps uniquely (M1) to the number of genomic locations where some reads map (M\\\
    _distinct). A PBC1 value in the range 0 - 0.5 is severe bottlenecking, 0.5 - 0.8\
    \ is moderate bottlenecking, 0.8 - 0.9 is mild bottlenecking, and > 0.9 is no\
    \ bottlenecking. PBC1 value > 0.9 is recommended, but > 0.8 is acceptable. ENCODE\
    \ processed alignments file {ENCFF994CYR|/files/ENCFF994CYR/} processed by ChIP-seq\
    \ ENCODE3 hg19 pipeline was generated from a library with PBC1 value of 0.64.\n\
    \n**audit\\_warning**: PBC2 (PCR Bottlenecking Coefficient 2, M1/M2) is the ratio\
    \ of the number of genomic locations where exactly one read maps uniquely (M1)\
    \ to the number of genomic locations where two reads map uniquely (M2). A PBC2\
    \ value in the range 0 - 1 is severe bottlenecking, 1 - 3 is moderate bottlenecking,\
    \ 3 - 10 is mild bottlenecking, > 10 is no bottlenecking. PBC2 value > 10 is recommended,\
    \ but > 3 is acceptable. ENCODE processed alignments file {ENCFF994CYR|/files/ENCFF994CYR/}\
    \ processed by ChIP-seq ENCODE3 hg19 pipeline was generated from a library with\
    \ PBC2 value of 3.12."
  labels:
    reference_genome: hg38/genomes/GRCh38-hg38
    accession: ENCSR474VGQ
    status: released
    assay_term_name: ChIP-seq
    simple_biosample_summary: ''
    biosample_summary: Homo sapiens KMS-11
    replication_type: isogenic
    biosample_ontology: /biosample-types/cell_line_EFO_0005694/
    perturbed: false
    doi: 10.17989/ENCSR474VGQ
    date_created: '2015-05-23T03:48:10.088276+00:00'
    date_released: '2017-01-25'
    submitter_comment: Files ENCFF199JVE, ENCFF133WUU had incorrectly identified paired_end
      1 and 2 metadata respectively. They have been corrected to match its fastq file
      flowcell signature accordingly.
    target: H3K27me3
