type: position_score
table:
  filename: ENCFF385WZD.tabix.bed.gz
  header_mode: none
  zero_based: true
  format: tabix
  chrom:
    column_index: 0
  pos_begin:
    column_index: 1
  pos_end:
    column_index: 2
scores:
- id: Histone_ChIP-seq_ENCSR000ANU
  column_index: 3
  type: float
  desc: 'Histone_ChIP-seq ENCSR000ANU [biosample_summary="Homo sapiens myotube originated from skeletal muscle myoblast" and target="H2AFZ"]'
  histogram:
    type: number
    number_of_bins: 100
meta:
  summary: 'Histone_ChIP-seq ENCSR000ANU [biosample_summary="Homo sapiens myotube originated from skeletal muscle myoblast" and target="H2AFZ"]'
  description: '**status**: released


    **biological\_replicates**: Rep 1, Rep 2


    **summary**: male adult (22 years)


    **output\_type**: replicated peaks


    **audit\_internal\_action**: Archived analysis {ENCAN475IFK|/analyses/ENCAN475IFK/}
    has in progress subobject quality standard {encode3-histone-chip|/quality-standards/encode3-histone-chip/}


    **audit\_warning**: Processed alignments file {ENCFF494ATJ|/files/ENCFF494ATJ/}
    processed by ChIP-seq ENCODE3 hg19 pipeline has 10831807 usable fragments. The
    minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting
    H2AFZ-human and investigated as a narrow histone mark is 10 million usable fragments.
    The recommended value is > 20 million, but > 10 million is acceptable. (See {ENCODE
    ChIP-seq data standards|/data-standards/chip-seq/})


    **audit\_warning**: Processed alignments file {ENCFF552VOM|/files/ENCFF552VOM/}
    processed by ChIP-seq ENCODE3 hg19 pipeline has 14717385 usable fragments. The
    minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting
    H2AFZ-human and investigated as a narrow histone mark is 10 million usable fragments.
    The recommended value is > 20 million, but > 10 million is acceptable. (See {ENCODE
    ChIP-seq data standards|/data-standards/chip-seq/})


    **audit\_warning**: PBC1 (PCR Bottlenecking Coefficient 1, M1/M\_distinct) is
    the ratio of the number of genomic locations where exactly one read maps uniquely
    (M1) to the number of genomic locations where some reads map (M\_distinct). A
    PBC1 value in the range 0 - 0.5 is severe bottlenecking, 0.5 - 0.8 is moderate
    bottlenecking, 0.8 - 0.9 is mild bottlenecking, and > 0.9 is no bottlenecking.
    PBC1 value > 0.9 is recommended, but > 0.8 is acceptable. ENCODE processed alignments
    file {ENCFF494ATJ|/files/ENCFF494ATJ/} processed by ChIP-seq ENCODE3 hg19 pipeline
    was generated from a library with PBC1 value of 0.85.


    **audit\_warning**: PBC2 (PCR Bottlenecking Coefficient 2, M1/M2) is the ratio
    of the number of genomic locations where exactly one read maps uniquely (M1) to
    the number of genomic locations where two reads map uniquely (M2). A PBC2 value
    in the range 0 - 1 is severe bottlenecking, 1 - 3 is moderate bottlenecking, 3
    - 10 is mild bottlenecking, > 10 is no bottlenecking. PBC2 value > 10 is recommended,
    but > 3 is acceptable. ENCODE processed alignments file {ENCFF494ATJ|/files/ENCFF494ATJ/}
    processed by ChIP-seq ENCODE3 hg19 pipeline was generated from a library with
    PBC2 value of 6.56.'
  labels:
    reference_genome: hg38/genomes/GRCh38-hg38
    accession: ENCSR000ANU
    status: released
    assay_term_name: ChIP-seq
    simple_biosample_summary: male adult (22 years)
    biosample_summary: Homo sapiens myotube originated from skeletal muscle myoblast
    replication_type: isogenic
    biosample_ontology: /biosample-types/in_vitro_differentiated_cells_CL_0002372/
    perturbed: false
    doi: 10.17989/ENCSR000ANU
    date_created: '2013-11-07T22:16:14.178626+00:00'
    date_released: '2011-02-10'
    submitter_comment: ''
    target: H2AFZ
