type: position_score
table:
  filename: ENCFF823GUD.tabix.bed.gz
  header_mode: none
  zero_based: true
  format: tabix
  chrom:
    column_index: 0
  pos_begin:
    column_index: 1
  pos_end:
    column_index: 2
scores:
- id: DNase-seq_ENCSR536VAY
  column_index: 3
  type: float
  desc: signal
  histogram:
    type: number
    number_of_bins: 100
meta:
  summary: 'DNase-seq ENCSR536VAY [TISSUE: Homo sapiens stimulated activated CD8-positive,
    alpha-beta memory T cell male adult (38 years) treated with anti-CD3 and anti-CD28
    coated beads for 24 hours, 100 ng/mL Interleukin-12 subunit alpha for 24 hours,
    100 ng/mL Interleukin-12 subunit beta for 24 hours]'
  description: '**status**: released


    **biological\_replicates**: Rep 1


    **summary**: male adult (38 years) treated with anti-CD3 and anti-CD28 coated
    beads for 24 hours, 100 ng/mL Interleukin-12 subunit alpha for 24 hours, 100 ng/mL
    Interleukin-12 subunit beta for 24 hours


    **output\_type**: peaks


    **audit\_error**: Alignment file {ENCFF581VZB|/files/ENCFF581VZB/} processed by
    DNase-seq ENCODE4 v3.0.0-alpha.2 GRCh38 pipeline ( {ENCPL848KLD|/pipelines/ENCPL848KLD/}
    ) for GRCh38 assembly has 12988014 mapped reads. According to ENCODE standards,
    conventional DNase-seq profile requires a minimum of 20 million uniquely mapped
    reads to generate a reliable SPOT (Signal Portion of Tags) score. The recommended
    value is > 50 million. For deep, foot-printing depth DNase-seq 150-200 million
    uniquely mapped reads are recommended. (See {ENCODE DNase-seq data standards|/data-standards/dnase-seq-encode4/})


    **audit\_internal\_action**: Released analysis {ENCAN118BBG|/analyses/ENCAN118BBG/}
    has in progress subobject document {1a06e1b2-e3d5-4355-8ab7-4a1fda29073a|/documents/1a06e1b2-e3d5-4355-8ab7-4a1fda29073a/}


    **audit\_internal\_action**: Released analysis {ENCAN118BBG|/analyses/ENCAN118BBG/}
    has in progress subobject document {835c42cc-f47e-4c47-af96-6d56313fd9e5|/documents/835c42cc-f47e-4c47-af96-6d56313fd9e5/}'
  labels:
    reference_genome: hg38/genomes/GRCh38-hg38
    accession: ENCSR536VAY
    status: released
    assay_term_name: DNase-seq
    simple_biosample_summary: male adult (38 years) treated with anti-CD3 and anti-CD28
      coated beads for 24 hours, 100 ng/mL Interleukin-12 subunit alpha for 24 hours,
      100 ng/mL Interleukin-12 subunit beta for 24 hours
    biosample_summary: Homo sapiens stimulated activated CD8-positive, alpha-beta
      memory T cell male adult (38 years) treated with anti-CD3 and anti-CD28 coated
      beads for 24 hours, 100 ng/mL Interleukin-12 subunit alpha for 24 hours, 100
      ng/mL Interleukin-12 subunit beta for 24 hours
    replication_type: unreplicated
    biosample_ontology: /biosample-types/primary_cell_NTR_0000669/
    perturbed: false
    doi: 10.17989/ENCSR536VAY
    date_created: '2020-04-20T23:45:28.483942+00:00'
    date_released: '2020-09-28'
    submitter_comment: ''
