type: position_score
table:
  filename: ENCFF977PNH.tabix.bed.gz
  header_mode: none
  zero_based: true
  format: tabix
  chrom:
    column_index: 0
  pos_begin:
    column_index: 1
  pos_end:
    column_index: 2
scores:
- id: DNase-seq_ENCSR261RWJ
  column_index: 3
  type: float
  desc: signal
  histogram:
    type: number
    number_of_bins: 100
meta:
  summary: 'DNase-seq ENCSR261RWJ [TISSUE: Homo sapiens Peyer''s patch tissue female
    adult (51 years)]'
  description: '**status**: released


    **biological\_replicates**: Rep 1


    **summary**: female adult (51 years)


    **output\_type**: peaks


    **audit\_internal\_action**: Archived analysis {ENCAN921VLI|/analyses/ENCAN921VLI/}
    has in progress subobject quality standard {encode3-dnase|/quality-standards/encode3-dnase/}


    **audit\_warning**: Alignment file {ENCFF732ROP|/files/ENCFF732ROP/} processed
    by DNase-seq ENCODE3 GRCh38 pipeline ( {ENCPL202DNS|/pipelines/ENCPL202DNS/} )
    for GRCh38 assembly has 39488148 mapped reads. According to ENCODE standards,
    conventional DNase-seq profile requires a minimum of 20 million uniquely mapped
    reads to generate a reliable SPOT (Signal Portion of Tags) score. The recommended
    value is > 50 million. For deep, foot-printing depth DNase-seq 150-200 million
    uniquely mapped reads are recommended. (See {ENCODE DNase-seq data standards|/data-standards/dnase-seq/})


    **audit\_warning**: Signal Portion of Tags (SPOT) is a measure of enrichment,
    analogous to the commonly used fraction of reads in peaks metric. ENCODE processed
    alignment files {ENCFF732ROP|/files/ENCFF732ROP/} processed by DNase-seq ENCODE3
    GRCh38 pipeline ({ENCPL202DNS|/pipelines/ENCPL202DNS/}) for GRCh38 assembly  have
    a SPOT1 score of 0.39. According to ENCODE standards, SPOT1 score of 0.4 or higher
    is considered a product of high quality data. Any sample with a SPOT1 score <0.3
    should be targeted for replacement with a higher quality sample, and a SPOT1 score
    of 0.25 is considered minimally acceptable SPOT1 score of 0.25 is considered minimally
    acceptable for rare and hard to find primary tissues. (See {ENCODE DNase-seq data
    standards|/data-standards/dnase-seq/})'
  labels:
    reference_genome: hg38/genomes/GRCh38-hg38
    accession: ENCSR261RWJ
    status: released
    assay_term_name: DNase-seq
    simple_biosample_summary: female adult (51 years)
    biosample_summary: Homo sapiens Peyer's patch tissue female adult (51 years)
    replication_type: unreplicated
    biosample_ontology: /biosample-types/tissue_UBERON_0001211/
    perturbed: false
    doi: 10.17989/ENCSR261RWJ
    date_created: '2019-08-18T16:57:12.319736+00:00'
    date_released: '2019-11-08'
    submitter_comment: ''
