| Id: | DNase-seq/ENCSR000EQH |
| Type: | position_score |
| Version: | 0 |
| Summary: |
DNase-seq ENCSR000EQH [biosample_summary="Homo sapiens T-helper 2 cell female adult (26 years)"] |
| Description: |
status: released biological_replicates: Rep 1 summary: female adult (26 years) output_type: peaks audit_warning: Alignment file {ENCFF615FBZ|/files/ENCFF615FBZ/} processed by DNase-seq ENCODE4 v3.0.0-alpha.2 GRCh38 pipeline ( {ENCPL848KLD|/pipelines/ENCPL848KLD/} ) for GRCh38 assembly has 28079837 mapped reads. According to ENCODE standards, conventional DNase-seq profile requires a minimum of 20 million uniquely mapped reads to generate a reliable SPOT (Signal Portion of Tags) score. The recommended value is > 50 million. For deep, foot-printing depth DNase-seq 150-200 million uniquely mapped reads are recommended. (See {ENCODE DNase-seq data standards|/data-standards/dnase-seq-encode4/}) audit_warning: Signal Portion of Tags (SPOT) is a measure of enrichment, analogous to the commonly used fraction of reads in peaks metric. ENCODE processed alignment files {ENCFF615FBZ|/files/ENCFF615FBZ/} processed by DNase-seq ENCODE4 v3.0.0-alpha.2 GRCh38 pipeline ({ENCPL848KLD|/pipelines/ENCPL848KLD/}) for GRCh38 assembly have a SPOT1 score of 0.29. According to ENCODE standards, SPOT1 score of 0.4 or higher is considered a product of high quality data. Any sample with a SPOT1 score <0.3 should be targeted for replacement with a higher quality sample, and a SPOT1 score of 0.25 is considered minimally acceptable SPOT1 score of 0.25 is considered minimally acceptable for rare and hard to find primary tissues. (See {ENCODE DNase-seq data standards|/data-standards/dnase-seq-encode4/}) |
| Labels: |
|
| ID | Type | Default annotation | Description | Histogram | Range |
|---|---|---|---|---|---|
| DNase-seq_ENCSR000EQH | float |
DNase-seq_ENCSR000EQH |
DNase-seq ENCSR000EQH [biosample_summary="Homo sapiens T-helper 2 cell female adult (26 years)"]
|
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[0.322, 28.5] |
| Filename | Size | md5 |
|---|---|---|
| ENCFF893DCZ.bed.gz | 452.34 KB | b058326896542fa1e20a1f947adabe91 |
| ENCFF893DCZ.bed.gz.dvc | 100.0 B | 38d4b60d27c96eddedfbc0c93e1eb76b |
| ENCFF893DCZ.tabix.bed.gz | 403.39 KB | b403e4e3328a028337842b09f976a229 |
| ENCFF893DCZ.tabix.bed.gz.dvc | 106.0 B | 72684db8d55748c8b9b9db1995569be3 |
| ENCFF893DCZ.tabix.bed.gz.tbi | 249.25 KB | c053d1f8591841da168a0111fe6470a8 |
| ENCFF893DCZ.tabix.bed.gz.tbi.dvc | 110.0 B | 00bec757a034a532ed89078c5d95c3d9 |
| genomic_resource.yaml | 2.65 KB | 900f753e2ec501a26ccafb445ce480bb |
| genomic_resource_original.yaml | 2.56 KB | deb6bbc33f1bdd1bba1d7fa929958056 |
| statistics/ |