type: position_score
table:
  filename: ENCFF245IFS.tabix.bed.gz
  header_mode: none
  zero_based: true
  format: tabix
  chrom:
    column_index: 0
  pos_begin:
    column_index: 1
  pos_end:
    column_index: 2
scores:
- id: DNase-seq_ENCSR000ELH
  column_index: 3
  type: float
  desc: signal
  histogram:
    type: number
    number_of_bins: 100
meta:
  summary: 'DNase-seq ENCSR000ELH [TISSUE: Homo sapiens keratinocyte female]'
  description: '**status**: released


    **biological\_replicates**: Rep 1


    **summary**: female


    **output\_type**: peaks


    **audit\_internal\_action**: Released analysis {ENCAN529UAX|/analyses/ENCAN529UAX/}
    has in progress subobject document {39983f73-4e70-46f2-88e4-e9fd0660fc73|/documents/39983f73-4e70-46f2-88e4-e9fd0660fc73/}


    **audit\_warning**: Alignment file {ENCFF117RNM|/files/ENCFF117RNM/} processed
    by DNase-seq ENCODE4 v3.0.0 GRCh38 pipeline ( {ENCPL848KLD|/pipelines/ENCPL848KLD/}
    ) for GRCh38 assembly has 35765456 mapped reads. According to ENCODE standards,
    conventional DNase-seq profile requires a minimum of 20 million uniquely mapped
    reads to generate a reliable SPOT (Signal Portion of Tags) score. The recommended
    value is > 50 million. For deep, foot-printing depth DNase-seq 150-200 million
    uniquely mapped reads are recommended. (See {ENCODE DNase-seq data standards|/data-standards/dnase-seq-encode4/})


    **audit\_warning**: Signal Portion of Tags (SPOT) is a measure of enrichment,
    analogous to the commonly used fraction of reads in peaks metric. ENCODE processed
    alignment files {ENCFF117RNM|/files/ENCFF117RNM/} processed by DNase-seq ENCODE4
    v3.0.0 GRCh38 pipeline ({ENCPL848KLD|/pipelines/ENCPL848KLD/}) for GRCh38 assembly  have
    a SPOT1 score of 0.27. According to ENCODE standards, SPOT1 score of 0.4 or higher
    is considered a product of high quality data. Any sample with a SPOT1 score <0.3
    should be targeted for replacement with a higher quality sample, and a SPOT1 score
    of 0.25 is considered minimally acceptable SPOT1 score of 0.25 is considered minimally
    acceptable for rare and hard to find primary tissues. (See {ENCODE DNase-seq data
    standards|/data-standards/dnase-seq-encode4/})'
  labels:
    reference_genome: hg38/genomes/GRCh38-hg38
    accession: ENCSR000ELH
    status: released
    assay_term_name: DNase-seq
    simple_biosample_summary: female
    biosample_summary: Homo sapiens keratinocyte female
    replication_type: unreplicated
    biosample_ontology: /biosample-types/primary_cell_CL_0000312/
    perturbed: false
    doi: 10.17989/ENCSR000ELH
    date_created: '2014-01-30T18:52:58.821150+00:00'
    date_released: '2011-04-19'
    submitter_comment: ''
